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. 2011 Feb 25;18(8):1366–1375. doi: 10.1038/cdd.2011.12

Figure 1.

Figure 1

Generation of the conditional knockout allele of mouse hausp gene. (a) Diagram of the hausp genomic region centering on exon 6 is shown, with restriction fragments of BamHI and HindIII indicated. WT, wild-type locus. Targeting vector contains exon 6 flanking with loxP sites and 7 kb 5′ homology and 3 kb 3′ homology. TV, targeting vector. Diagram of the conditional knockout allele shows the altered restriction fragments of BamHI and HindIII after homologous recombination. Floxed, exon 6-floxed conditional knockout allele. (b) Genotyping was determined by Southern blot using genomic DNA digested with BamHI. Hybridization with 5′ external probe detected a 10 kb band in wild-type mice (+/+) and an additional 8 kb band for the FL in heterozygote mice (+/fl). Only the 8 kb band for the FL was detected in hausp conditional knockout homozygote mice (fl/fl). (c) Genotyping was determined using three mixed primers to detect wild-type allele and the FL simultaneously to identify wild-type mice (+/+), heterozygote mice (+/fl), and homozygote mice (fl/fl)