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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Microbiol. 2011 Jun 16;81(2):554–570. doi: 10.1111/j.1365-2958.2011.07715.x

Figure 3. Strain PAO1 shows a decreased level of HA-MucA and increased MucD expression on PIA-AMV.

Figure 3

A. Western blot analysis was performed as previously described (Damron et al., 2009b). Cell lysates were prepared from cells after 24 hr growth on PIA and PIA-AMV. The membranes were probed with anti-HA (Roche) and anti-alpha subunit of RNAP antibodies (Neoclone) as a loading control. Shown are representative panels of blots from three independent experiments with 50μg of total lysate. Positions of apparent molecular masses are indicated aligned with a schematic of HA-tagged MucA C. Total protein lysates of PAO1 cultured on PIA and PIA-AMV as well as a mucD mutant strain, were separated by SDS-PAGE and transferred to nitrocellulose membranes. Membranes were probed sequentially with anti-MucD (Wood & Ohman, 2006) and anti-alpha RNA polymerase (Neoclone). MucD signal was normalized to 1 for PAO1 on PIA for comparison to PAO1 on PIA-AMV. Shown is a representative of three independent experiments with standard deviation indicated for biological replicates.