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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Microbiol. 2011 Jun 3;81(2):540–553. doi: 10.1111/j.1365-2958.2011.07713.x

Fig. 5.

Fig. 5

The SecM signal peptide specifies intermediate targeting kinetics. MC4100 were transformed with a plasmid (pHL36, pMY10-11) encoding the indicated HA-tagged OmpA derivative. Cells were subjected to pulse-chase labeling after the addition of IPTG and immunoprecipitations were performed using anti-HA and anti-OmpC antisera. p, precursor; m, mature. A separate aliquot of cells transformed with pHL36 was removed and treated with 2 mM sodium azide (NaN3) 2 min prior to labeling to show the position of the OmpC precursor.