Figure 5.

Cytokine responses in the culture supernatants of spleen cells stimulated for 72 hours with rBmVAL-1 (A) rBmALT-2 (B), or rBmVAL-1-BmALT-2 hybrid protein (C) were measured using an ELISA. Concentrations of each cytokines are represented as pg/mL; spleen cells from vector/alum injected mice served as control. Additional controls include cells incubated in media alone and cells stimulated with a non-specific recombinant protein. Spleen cells from the prime boost group were used for the last two control groups; N = 10.

Note: *Significance P < 0.01 compared with control cells.