Fig. 2.

Tregs generated from yeast coculture exhibit decreased suppressive function on CD4⁺CD25⁻ effector cells compared to Tregs generated from CD40L coculture. A representative histogram shows the suppression of CD4⁺CD25⁻ T-cell proliferation by CD4⁺CD25⁺CD127⁻ Tregs in a healthy donor. CD4⁺CD25⁺CD127⁻ Tregs generated from yeast and CD40L cocultures were isolated by Miltenyi columns and rested for 24 h. Tregs were then cultured alone or cocultured at a 1:1 ratio with autologous CD4⁺CD25⁻ T cells, upon stimulation with anti-CD3 and irradiated autologous T-depleted PBMCs. Experiments were performed in triplicate and results are expressed as the mean ± SD (cpm). Percentages indicate the level of proliferation.