Skip to main content
. Author manuscript; available in PMC: 2011 Jul 12.
Published in final edited form as: Dev Cell. 2009 Feb;16(2):314–320. doi: 10.1016/j.devcel.2009.01.001

Figure 3. Increased chromatin accumulation of monoubiquitinated Fancd2 and impaired Fancd2 foci assembly in Usp1-deficient MEFs.

Figure 3

(A) Wild-type and Usp1−/− MEFs were either left untreated or treated with UV (30 J/m2). Cells were collected 3 hr after UV irradiation and fractionated. Each fraction was immunoblotted with the indicated antibodies. (B and C) Wild-type and Usp1−/− MEFs were stained for Fancd2 following either mock treatment (untreated) or 500 ng/ml of MMC for 20 hr. Bar, 10 μm (B) and 5 μm (C). (D) Quantification of cells with Fancd2 foci. Values represent the mean ± SEM, examined at least 700 nuclei each in three independent experiments. (E) Wild-type (upper panels) and Usp1−/− (lower panels) MEFs were stained for γ-H2AX and 53BP1 foci following MMC treatment (500 ng/ml) for 20 hr. Bar, 10 μm.