Figure 4.
(A) Global H/DX titration experiments: CaM titrated with Ca2+ (squares), 2.9:1 melittin:CaM titrated with Ca2+ (diamonds), and 2.9:1 mastoparan:CaM titrated with Ca2+ (circles). H/DX was conducted at a constant 10 min with 97% D2O, 10 mM HEPES (pH 7.4), 150 mM KCl, and 4–5 M CaM. (B) Output of the PLIMSTEX titration modeling using a five parameter search including D0, ΔD3, ΔD4, β3, and β4.