Skip to main content
. Author manuscript; available in PMC: 2011 Jul 12.
Published in final edited form as: Nat Neurosci. 2009 Feb 22;12(3):311–317. doi: 10.1038/nn.2275

Figure 1. MeCP2 is present in all glial cell types in normal rat and mouse brains.

Figure 1

(a) Immunostaining showing MeCP2 is present in nuclei of cultured rat glia. MeCP2 protein (green) and cell-specific marker proteins (red) are indicated. Calibration bar, 20 μm. (b) Real time RT-PCR analysis showing MeCP2 mRNA levels in rat glia. MeCP2 transcripts in cortical neurons are shown for comparison. Error bars represent standard deviation (SD) based on three independent experiments. (c) Western blot analysis showing MeCP2 protein in rat glia (left panel) and optic nerve (right panel). MeCP2 and Sin3A migrate at 75 kDa and 150kDa, respectively. (d) Co-immunostaining of rat or mouse brain sections for MeCP2 (red) and the glial-specific markers (green) as indicated. Calibration bars, 20 μm.