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. Author manuscript; available in PMC: 2011 Jul 12.
Published in final edited form as: Nat Neurosci. 2009 Feb 22;12(3):311–317. doi: 10.1038/nn.2275

Figure 2. MeCP2 is detected in astrocytes in brain sections from wild-type but not MeCP2-null mice.

Figure 2

(a) Co-immunostaining of brain sections from 6-week-old wild-type (+/y) and RTT (−/y) mice for MeCP2 (red) and GFAP (green). Arrows indicate the presence and absence of MeCP2 in astrocyte nuclei of wild-type (WT) and RTT brains, respectively. Calibration bars, 40 μm. (b) Western blot analysis confirms the presence of MeCP2 in WT and its absence in MeCP2-null astrocytes. Arrow indicates MeCP2 C-terminal peptide product of the recombination event in the Jaenisch mouse model. Sin3A serves as loading control. (c) Western blot showing an altered global chromatin signature in astrocytes from RTT mice (Mut). Histones were probed with the indicated antibodies to histone modifications.