Table 3.

Expression pattern consistent miRNA tags in microarray profiling and high-throughput sequencing under drought stress

Tag name	Sequence (5'–3')	Microarray			High-throughput sequencing
		Group C median signal	Control median signal	log2 (Group C/control)	Control expressed	Group C expressed	Control normalized	Group C normalized	log2 (Group C/control)
ath-miR156g	CGACAGAAGAGAGUGAGCAC	6106.99	4801.66	0.35	106.	72	22.07	40.18	0.86
ath-miR319a	UUGGACUGAAGGGAGCUCCCU	9658.96	6994.93	0.41	19	35	3.96	19.53	2.30
bna-miR156a	UGACAGAAGAGAGUGAGCACA	4760.84	3484.23	0.47	7229	9584	1505.19	5348.95	1.83
ghr-miR156c	UGUCAGAAGAGAGUGAGCAC	4302.29	3028.75	0.59	15	23	3.12	12.84	2.04
peu-miR102	UCUUUCCGAGUCCUCCCAUACC	3937.86	3270.72	0.27	73	72	15.20	40.18	1.40
peu-miR102*	UAUGGGAGAGGCGGGAAUGACU	665.89	310.08	1.10	8	15	1.67	8.37	2.33
peu-miR123a	UGUCGCAGGAGAGAUGGCGCU	302.07	109.51	1.59	515	387	107.23	215.99	1.01
peu-miR123b	UGUCGCAGGAGAGAUGGCGCUA	263.11	88.76	1.60	588	344	122.43	191.99	0.65
peu-miR129	UUCAUUCCUCUUCCUAAAAUGG	248.30	64.90	1.88	89	87	18.53	48.56	1.39
pta-miR319	UUGGACUGAAGGGAGCUCC	8945.88	6395.55	0.49	4	12	1.00	6.70	2.74
ptc-miR156a	UGACAGAAGAGAGUGAGCAC	6205.64	4932.89	0.41	122451	109321	25496.16	61013.36	1.26
ptc-miR156k	UGACAGAAGAGAGGGAGCAC	3549.59	2359.96	0.68	439	248	91.41	138.41	0.60
ptc-miR162a	UCGAUAAACCUCUGCAUCCAG	9416.06	6869.24	0.45	3671	1859	764.36	1037.53	0.44
ptc-miR167f	UGAAGCUGCCAGCAUGAUCUU	8097.06	6035.68	0.41	3202	1616	666.71	901.91	0.44
ptc-miR319a	UUGGACUGAAGGGAGCUCCC	12057.19	10260.72	0.23	67	183	13.95	102.13	2.87
ptc-miR396a	UUCCACAGCUUUCUUGAACUG	4357.86	1427.49	1.68	186	352	38.73	196.46	2.34
ptc-miR396c	UUCCACAGCUUUCUUGAACUU	542.90	83.90	2.66	92	110	19.16	61.39	1.68
ptc-miR473a	ACUCUCCCUCAAGGCUUCCA	2335.32	1671.64	0.52	1153	2216	240.07	1236.78	2.37
sbi-miR156e	UGACAGAAGAGAGCGAGCAC	4269.41	3034.67	0.53	118	81	24.57	45.21	0.88
vvi-miR156e	UGACAGAGGAGAGUGAGCAC	3387.98	2394.35	0.51	52	47	10.83	26.23	1.28
vvi-miR167c	UGAAGCUGCCAGCAUGAUCUC	4819.80	2951.66	0.77	27	23	5.62	12.84	1.19
ptc-miR166n	UCGGACCAGGCUUCAUUCCUU	2540.95	4532.28	–0.89	799	33	166.36	18.42	–3.18
ptc-miR169z	CAGCCAAGAAUGAUUUGCCGG	8.87	69.20	–2.96	320	59	66.63	32.93	–1.02

‘Group C/control median signal’, the median signal for each of the three miRNA tags probe in the microarray. ‘Group C/control expressed’, the high-throughput sequencing reads for each miRNA tags; ‘Group C/control normalized’, the normalized miRNA tag expression in tags per million; ‘log₂ (GroupC/control)’, log₂ ratio of the median signal in the microarray or the normalized expression in the high-throughput sequencing between drought treatment (Group C) and control samples.