Fig. 3.

AtCBF3 transcript levels in S. tuberosum (St) and S. commersonii (Sc) transformed with 35S::AtCBF3. AtCBF3 transcript levels were determined by qRT-PCR in three Sc and two St 35S::AtCBF3 transgenic lines grown at 25 °C. qRT-PCR was performed using 100 ng of total RNA per sample. Relative expression was calculated using the potato 60S gene (clone STMCK67) as an internal reference. Relative expression of Sc 21 was set to 1 and the other lines were adjusted accordingly. The letters a, b, and c indicate statistically significant differences (ANOVA, P <0.05, n=2). Error bars indicate the standard deviation. WT, non-transformed plants.