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. 2011 Apr 21;62(11):3807–3819. doi: 10.1093/jxb/err066

Fig. 6.

Fig. 6.

Transcript accumulation of S. commersonii (Sc) and S. tuberosum (St) orthologues of CBF1, ZAT10, RAV1, and CZF1 in response to mechanical agitation. Three-week-old plants grown in magenta boxes were mechanically agitated for 15 min (see Materials and methods). Tissue was collected from two plants at 0, 15, and 30 min after the mechanical agitation treatment. The control sample received no mechanical agitation. qRT-PCR analysis was performed to determine the transcript levels of Sc and St genes. Average values of two different experiments are shown. The relative expression level of each transcript was normalized using the potato 60S gene (clone STMCK67) as an internal reference. The relative expression of the Sc 0 min sample was set to 1 and the other values were adjusted accordingly. Error bars indicate the SE.