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. 2011 Apr 21;62(11):3875–3884. doi: 10.1093/jxb/err078

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — Accumulation of NO in Fe-deficient roots and effects of NO availability on root branching. (a) Photographs of nitric oxide production shown as green fluorescence in representative roots. (b) The NO production expressed as the fluorescence intensity relative to the root tips of +Fe plants. (c) The lateral root number on the 15 cm root tip. The tomato plants were grown in nutrient solution that contained (+Fe) or omitted (−Fe) Fe-EDTA. For pharmacological treatment, either 100 μM NONOate (+Fe+NONOate) or 100 μM SNP (+Fe+SNP) were added to the +Fe solution, and 200 μM cPTIO (–Fe+cPTIO) was added to the –Fe solution. The NO levels and the lateral roots numbers were analysed on the 6th day of treatments. Data are means ±SD (n=8). Different letters indicate significant differences (P <0.05) between treatments.