Fig. 1.

35S–GFP–ABI4 and 35S–ABI4–GUS confer hypersensitivity to ABA and salt stress. (A) Hypersensitivity to ABA and NaCl inhibition of germination due to 35S–GFP–ABI4 and 35S–ABI4–GUS transgenes in Col and rdr6 backgrounds, respectively. Germination was scored as radicle emergence after 4 d of incubation on minimal nutrient salt medium (min), or min supplemented with 1 μM ABA or 200 mM NaCl. (B) 35S–ABI4–GUS confers hypersensitivity to ABA for inhibition of root growth in rdr6 background. Root lengths were measured 6 d after transfer from GM to fresh GM with or without 3 μM ABA. Genotypes are indicated by genetic background (Col or rdr6) and transgene present (none, 35S–GFP–ABI4, or 35S–ABI4–GUS).