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. 2011 May 4;62(11):4055–4065. doi: 10.1093/jxb/err112

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — Subcellular distribution and Ca²⁺ response of native Ntann12 proteins. The total protein extract from roots of 4-week-old tobacco plants was treated with either CaCl₂ or EDTA before separation of the membrane-enriched and cytosolic fractions and western blot analysis using the anti-Ntann12 antibodies. (A) Cytosolic fraction. (B) Membrane-enriched fraction. For the analysis, 40 μg of membrane-enriched fraction proteins and 60 μg of cytosolic fraction proteins were loaded into gels. M, molecular marker. The arrow denotes the position of the expected size for Ntann12.