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. 2011 May 4;62(11):4055–4065. doi: 10.1093/jxb/err112

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — Ntann12 immunolocalization in tobacco root sections visualized by fluorescence microscopy. (A–D) A 100μm cross-section. (E–H) A 100 μm longitudinal section. (I–L) A 50–70 nm cross-section. (M–O) A 100μm cross-section. (A), (E), (I), (M) Phase contrast. (B), (F), (J) Staining with 4',6-diamidine 2-phenyl indole (DAPI). (C), (G), (K) Immunostaining with affinity-purified antiserum against Ntann12. (D), (H), (L) Superposition of DAPI staining and immunostaining with affinity-purified antiserum against Ntann12. The green fluorescence is indicative of the presence of Ntann12, whereas DNA is represented by blue colour resulting from DAPI staining. (N), (O) The green fluorescence shows the background autofluorescence without immunostaining. Bar=50 μm (A–H and M–O), 5 μm (I–L).