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Cell targeting with NPB-AF647. a) Flow cytometry analysis of Ramos and Jurkat cells treated with NPB-AF647 after pretreatment with different FPs or receiving no FP pretreatment. Ramos and Jurkat cells receiving neither FP nor NP treatment are given as references (right column). b) Dependence of NPB-AF647 cell-binding on FP pre-labeling, demonstrated by flow cytometry, where the secondary antibody, goat-antimouse-Cy3 (GAM-Cy3), indicates FP labeling.
