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Overexpressing a truncated clone of NRG1 suppresses snf2Δ. Yeast strains FY32 (snf2Δ1::HIS3 SUC2) and yHZ269 (snf2Δ1::HIS3 SUC2-36) were transformed with nrglΔZn or full-length NRG1 cloned in pRS426, as well as vector alone. Ura+ single colonies carrying each construct were resuspended in 200 μl sterile water, and spotted on SC-Ura plates containing glucose or raffinose as the carbon source. Plates were photographed on day 2.
