Figure 5. Time course for optimum gene knockdown after soaking snails in either dsRNA/PEI or siRNA/PEI nanoparticles.
(A) Relative expression of Cathepsin B gene in juvenile snails soaked for between 1 and 4 days in CathB dsRNA/PEI. (B) Relative expression of peroxiredoxin gene in snails soaked for between 1 and 4 days in Prx dsRNA/PEI. (C) Relative expression of Cathepsin B gene in snails soaked for 1 to 4 days in CathB1-siRNA/PEI. Note in all time course studies that the optimum knockdown of transcripts occurs at day 3 of incubating in either dsRNA/PEI or siRNA/PEI complexes.