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. 2011 Jul 12;6(7):e21359. doi: 10.1371/journal.pone.0021359

Figure 3. Successful completion of strand exchange reactions in the presence of ATPγS.

Figure 3

(a). Using SDS to remove RecA from nucleoprotein filaments in order to verify the completion of strand exchange reaction in the “invading strand” experiment. (i). Tethered beads disappear after SDS addition if the RecA-mediated strand exchange reaction did not proceed. (ii). Tethered beads remain and the bead Brownian motion amplitude approaches the expected product value after SDS addition, if strand exchange reaction is successful. (b). A successful, representative time-course using 427/352 nt hybrid DNA, 427 nt homologous ssDNA and ATPγS. The tether remained after SDS addition, and the Brownian motion approaches the expected 427 bp duplex DNA value (50.3±6.5 nm).