|
K. pneumoniae
strains
|
|
|
| NTUH-K2044 |
Clinical isolate; the parent strain for generate isogenic mutants |
(18) |
| i3705 |
km cassette in wbaP ORF |
(15) |
| i3706 |
km cassette in wcaI ORF |
(15) |
| i3709 |
km cassette in wcaG ORF |
(15) |
| i3714 |
km cassette in wzy ORF |
(15) |
| i3715 |
km cassette in ptf ORF |
(15) |
| d3695 |
glf deletion strain in NTUH K2044 background |
present study |
| d3699 |
uge deletion strain in NTUH K2044 background |
present study |
| d3704 |
gnd deletion strain in NTUH K2044 background |
present study |
| d3705 |
wbaP deletion strain in NTUH K2044 background |
present study |
| d3706 |
wcaI deletion strain in NTUH K2044 background |
present study |
| d3708 |
wcaH deletion strain in NTUH K2044 background |
present study |
| d3709 |
wcaG deletion strain in NTUH K2044 background |
present study |
| d3712 |
atf deletion strain in NTUH K2044 background |
present study |
| d3713 |
wcfG deletion strain in NTUH K2044 background |
present study |
| d3714 |
wzy deletion strain in NTUH K2044 background |
present study |
| d3715 |
ptf deletion strain in NTUH K2044 background |
present study |
| d3718 |
wzc deletion strain in NTUH K2044 background |
present study |
| d3719 |
wzb deletion strain in NTUH K2044 background |
present study |
| d3720 |
wza deletion strain in NTUH K2044 background |
present study |
| d3742 |
yegM deletion strain in NTUH K2044 background |
present study |
| c3706 |
complement of wcaI in d3706 |
present study |
| c3709 |
complement of wcaG in d3709 |
present study |
| c3712 |
complement of atf in d3712 |
present study |
|
E. coli
strains
|
|
|
| DH5α |
|
|
| BL21 star |
|
|
| BL21 codon plus |
|
|
|
Plasmids
|
|
|
| pET28a |
expression cloning |
Novagen |
| pGEM-T easy-km |
pGEM-T easy was inserted with Km cassette from pUC4K into NdeI site for trans complementation |
Present study |
| pKO3-km |
pKO3-derived plasmid, with an insertion of Km resistance cassette from pUC4K into AccI site |
(36) |
| pCRIITOPO-CAT |
for cloning the complement plasmid |
(15) |
