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MCF7 cells were transfected as in Figure 2A and B , fractionated to insoluble (A) and soluble (B) fractions, and then analyzed for expression of E-cadherin. Graphs represent E-cadherin normalized to β-actin in each fraction calculated from 3 independent experiments. Immunoblot represents a typical experiment. *, P<0.05, **, P<0.01, ***, P<0.001. Error bars represent SE.
