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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Arthritis Rheum. 2011 Aug;63(8):2473–2483. doi: 10.1002/art.30317

Figure 3. TGF-β1 induced Snail-1 expression in primary pulmonary EC.

Figure 3

A, B. Primary pulmonary EC were treated with TGF-β1 for 24 h, fixed and stained by indirect immunofluorescence with Snail-1. Note a marked increase in expression of Snail-1 following treatment with TGF-β. B. Fluorescence density of α-SMA expression analyzed using NIH Image J software. C. Primary pulmonary EC were treated with TGF-β1 for 72 h. RNA was purified and reverse transcribed and assessed by PCR for Snail-1 and Snail-2 expression. GAPDH was used as control. Note very low level basal expression of Snail-1. Treatment with TGF-β1 increased Snail-1 expression. In contrast, Snail-2 levels displayed higher constitutive expression and did not show any change following TGF-β treatment. D. Quantitative real-time PCR analysis of TGF-β1 induction of Snail-1 mRNA expression. The data represent the mean ± SD of one of two independent experiments in which each sample was analyzed in triplicate (*p < 0.05 when compared with controls). Size bar = 50μm