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. Author manuscript; available in PMC: 2012 Jul 19.
Published in final edited form as: Biochemistry. 2011 Jun 24;50(28):6196–6207. doi: 10.1021/bi2004284

Figure 8.

Figure 8

A) chymotrypsin digestion products of Cdc42 wild type and Cdc42(T35A). The intensity of the parent Cdc42 protein bands in lane 3 in both gels that were not treated with chymotrypsin (Incubation time with enzyme = 0) and assumed to 100% protein for each gel. Initial concentrations used for each protein were the same. In each gel, corresponding Cdc42 (wild type) or (T35A) bands were normalized to the parent band (Lane 3) at ~20 kDa (indicated by asterisk). Lanes 4–12 in each gel represents the chymotrypsin digestion products obtained after incubation with either Cdc42 or Cdc42(T35A) for 20, 40, 50, 60, 70, 80, 90, 100, and 110 minutes respectively. In each gel, lane 1 represents the MW marker, and lane 2 represents that of chymotrypsin alone to show corresponding bands from the autolysis of chymotrypsin. B) A graphical depiction of the % of undigested Cdc42 wild type (Circles) or Cdc42(T35A) (Squares) incubated with chymotrypsin as a function of time. Error bars represent the standard deviation of triplicate measurements.