Skip to main content
. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Oncogene. 2011 Apr 4;30(37):3943–3952. doi: 10.1038/onc.2011.105

Fig. 2. Blocking H3S28ph represses endogenous Pol III gene transcription.

Fig. 2

(A) EGF enhances Pol III gene transcription in JB6 cells. Cells were treated with 20 ng/ml EGF for 60 min and total RNAs were extracted from these cells. The pre-tRNALeu, pre-tRNATyr, 7SL RNA, 5S rRNA, and GAPDH transcripts were measured by RT-qPCR. The fold change was calculated by normalizing to the amount of GAPDH mRNA. (B) Inhibitor of EGFR represses the activity of pre-tRNALeu and 5S rRNA genes. The cells were pretreated with AG1478 for 1h and treated with EGF for 60 min. tRNALeu and 5S rRNA was determined by RT-qPCR. (C) Inhibition of H3S28ph reduces Pol III gene transcription. JB6 cells were transiently transfected with WT H3 and mutant H3S28A expression constructs for 48h and treated with EGF, the amounts of pre-tRNALeu, 5S rRNA, and GAPDH transcripts were measured by RT-qPCR. The bars represent means ± SE of at least three independent determinations.