Fig. 3. EGF enhances expression of TFIIIB subunits.
(A) EGF increases cellular levels of TFIIIB subunits in JB6 cells. JB6 cells were treated with EGF. Immunoblot analysis was performed using protein lysates derived from these cells and antibodies against Brf1, TBP, Bdp1, TFIIIC63 and β-actin as designated. A representative blot from three independent determinations is shown. (B) EGF enhances mRNA levels of Brf1, TBP, Bdp1 and TFIIIC63. JB6 cells were treated with EGF. The amounts of Brf1, TBP, Bdp1, TFIIIC63 and GAPDH transcription were measured by RT-qPCR. The values represent means ± SE of three independent determinations.
