Table 1.
HiTS-FLIP binding statistics.
k-mer | Mean measurements per k-mer1 | No. of k-mers with KD < 1 μM | Avg. standard error2 of log(KD) |
---|---|---|---|
8mers | 77,485 | 219 | 3.2% |
9mers | 18,791 | 1,113 | 3.1% |
10mers | 4,528 | 4,546 | 4.0% |
11mers | 1,091 | 19,268 | 6.4% |
12mers | 475 | 81,852 | 11.4% |
The number of clusters passing our quality filter and the total number of binding measurements were 88,052,302 and 440,261,510, respectively.
Standard error is an average over all k-mers having Kd < 1 μM and was calculated by treating 5 flow cell lanes as technical replicates and fitting a Kd for each.