Skip to main content
. Author manuscript; available in PMC: 2012 Jul 13.
Published in final edited form as: Structure. 2011 Jul 13;19(7):930–944. doi: 10.1016/j.str.2011.05.002

Figure 1. Sequences of Nova KH Domains and RNA Hairpin Target and the Structure of the Nova-1 KH1/2-RNA Hairpin Complex in the crystal.

Figure 1

(A) Schematic of three KH domains and intervening linkers in Nova proteins. Red-colored numbers indicate the length of linker segments in Nova-1.

(B) The Nova-1 KH1/2 construct used in the current project.

(C) The sequence of the in vitro selected 25-mer RNA hairpin. The tandem UCAN sites are colored in gold (U9 to G12) and cyan (U13 to C16). Structural studies were also undertaken on a sequence containing a 5BrU2•A24 pair.

(D) Sequence and secondary structure (Lewis et al, 1999) alignments of Nova KH1, KH2 and KH3 domains, with conserved residues shown in red. The GXXG motif and so-called variable loop, are denoted as I and V, respectively.

(E) Ribbon representation of the structure of the Nova-1 KH1/2-RNA hairpin complex in the crystal. The stoichiometry is KH1/2:RNA hairpin of 2:2. The color codes are RNA in green, KH1 in gold and KH2 in cyan. The UCAC motif interacting with KH1 is colored in blue.

See also Table S1, Figures S1, S2 and S3.