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. Author manuscript; available in PMC: 2012 Jul 13.
Published in final edited form as: Structure. 2011 Jul 13;19(7):930–944. doi: 10.1016/j.str.2011.05.002

Figure 5. Filter Binding Assays for Nova KH Domain-RNA Complexes.

Figure 5

(A) The sequence of the Nova-2 KH3-binding 20-nt RNA hairpin containing a single UCAN site (Lewis et al., 2000).

(B) The sequence of the Nova-1 KH1/2-binding 25-nt RNA hairpin containing a (UCAN)2 site (Musunuru and Darnell, 2004) that was used in this work.

(C) The sequence of the Nova-1 KH1/2/3-binding 32-nt RNA hairpin containing a (UCAN-N)3 site (Buckanovich and Darnell, 1997).

(D) Filter-binding assays for complex formation of Nova KH1/2/3 (open circles), KH1/2 (black circles), KH2 (red circles), and KH3 (green circles) with KH1/2 RNA hairpin containing a (UCAN)2 site (panel B).

(E) Filter-binding assays for complex formation of wild-type Nova KH1/2 (black circles), Ser14Glu mutant (green circles), and Lys40Gln, Lys43Gln dual mutant (red circles) with KH1/2 RNA hairpin containing a (UCAN)2 site (panel B).

(F) Filter-binding assays for complex formation of Nova KH1/2 with RNA hairpins containing a single UCAN site (panel A) (red circles), (UCAN)2 site (panel B) (black circles), and (UCAN-N)3 site (panel C) (green circles).

(G) Filter-binding assays for complex formation of Nova KH2 with RNA hairpins containing a single UCAN site (panel A) (red circles), (UCAN)2 site (panel B) (black circles), (UCAN-N)3 site (panel C) (green circles), and a RNA hairpin specific for FMRP KH2 domain (open circles).

(H) Filter-binding assays for complex formation of Nova KH3 with RNA hairpins containing a single UCAN site (panel A) (red circles), (UCAN)2 site (panel B) (black circles), and (UCAN-N)3 site (panel C) (green circles).