Table 2. NusA does not control rrnH transcription.
Shown are luciferase values (× 10−6). MDS42 and MDS42 mutants carrying an rrnH 16S - luc fusion were grown overnight in LB at 37°C (stationary phase), diluted 1:50, and incubated at 37°C to OD600 0.20 – 0.45 (log phase). Initial OD600 and viability were equivalent for the three strains. Shown is a representative experiment; assays were performed at least 4 times with equivalent results.
| strain | nus | Stationary | Log |
|---|---|---|---|
| 10916 | + | 1.2 | 30.5 |
| 10918 | nusA-ΔAR2 | 1.2 | 31.2 |
| 10935 | ΔnusA | 1.9 | 33.0 |