Figure 2. RIG-3 is expressed in ACh neurons.
(A) The mCherry-tagged rig-3 genomic construct is expressed in cholinergic motor neurons, which are identified by expression of the acr-2::gfp reporter. Arrows indicate the cholinergic neurons that express RIG-3. (B) Distribution of mCherry::RIG-3 (expressed with the rig-3 promoter) and GFP::SNB-1 (expressed in DA neurons with the unc-129 promoter) are compared in dorsal cord axons. (C) mCherry::RIG-3 fluorescence in coelomocytes is shown. (D) Soluble mCherry (expressed in cholinergic neurons) did not label coelomocytes.