Figure 2. Effect of Deleting LEPRs from VGAT+ and VGLUT2+ Neurons on Body Weight, Body Composition, and Food Intake.
(A) Body weight curves of male and female mice with LEPR deleted from VGAT+ neurons (open boxes), VGLUT2+ neurons (closed triangles) or controls (Leprlox/lox, closed circles). Data are presented as mean +/− SEM. Compared to the Leprlox/lox controls, all values are significantly different except in week 4 and 7 for the Vglut2-ires-Cre, Leprlox/lox females (assessed by one-way ANOVA with Dunnett’s test of all groups compared to Leprlox/lox mice ).
(B) Fat mass and lean mass of male and female Vgat-ires-Cre, Leprlox/lox mice (left) and Vglut2-ires-Cre, Leprlox/lox mice (right) at 10 weeks of age were analyzed by EchoMRI. Data are presented as mean +/− SEM.*, p< 0.05; ***, p<0.001; unpaired t-test compared to Leprlox/lox controls.
(C) Food intake of Vgat-ires-Cre, Leprlox/lox mice (left) and Vglut2-ires-Cre, Leprlox/lox mice (right). Data are presented as mean +/− SEM.***, p<0.001; unpaired t-test compared to Leprlox/lox controls.