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Comparison of standard curves for the inhibition of anti-3-nitrotyrosine antibody binding by various proteins in the C-ELISA. The curves show competition for the anti-3-nitrotyrosine antibody between the immobilized BSANT and competing free proteins in solution: ■ BSANT (n = 3); ● BSA (n = 2); ▲ KLH (n = 2). The percentage inhibition of maximum antibody binding (absorbance at 490 nm in the absence of competition) is plotted against the competing free protein concentration.
