Table 1.
Mean 3-nitrotyrosine levels in plasma from mussels (n = 20) before and after PMA-stimulation. 3-nitrotyrosine contents of plasma proteins were determined in triplicate by C-ELISA as described in the Material and Methods and expressed as BSANT equivalents. "unstimulated": plasma from unstimulated haemolymph, "+ PMA": plasma 30 min after addition of 10 μL PMA (1 mg mL-1) to haemolymph, "PMA + L-NIO": plasma after 5 min incubation of haemolymph with L-NIO and 30 min with PMA.
| Unstimulated | + PMA | + PMA + L-NIO |
| 0.037 ± 0.025 (n = 20) | 0.118 ± 0.024 (n = 20) | 0.082 ± 0.024 (n = 20) |