Table 2.

Reference chemicals tested in the intra-laboratory pre-validation of an in vitro angiogenesis assay.

Chemical name	CAS-RN	Chemical class	Product class	Concentrations tested	Purity	Supplier	Physical and chemical characteristics
Acetyl salicylic acid	50-78-2	Salicylate	Non-steroidal anti-inflammatory drug (NSAID)	10, 100, 500, 1000, 1500, and 2000 μM	99.9%	Sigma Aldrich	Powder
Erlotinib	183319-69-9	Quinazoline	HER1/EGFR tyrosine kinase inhibitor	0.0005, 0.001, 0.01, 0.1, 1, 10, 25, and 50 μM	99.9%	Roche Diagnostics	Powder
Levamisole	16595-80-5	Imidazothiazole	Alkaline phosphatase inhibitor	0.01, 0.1, 1, 10, 50, 100, 250, 500, 750, 1000, and 2000 μM	99%	Sigma Aldrich	Powder
2-Methoxyestradiol	363-07-2	Estradiol metabolite	Tubulin inhibitor	0.01, 0.1, 0.2, 0.4, 0.6 0.8, 1, and 2 μM	99.5%	Sigma Aldrich	Powder
Thalidomide	50-35-1	Phthalimide	Immunomodulator, TNF-α inhibitor	10, 100, 200, 300, 400, and 500 μM	>99%	Sigma Aldrich	Powder
Anti-VEGF	n/a	Human VEGF165 and VEGF121 antibody, IgG fraction of antiserum	Growth factor antibody	0.5, 1, 2.5, 5, 7.5, 10, 25, and 50 μg/ml	n/a	Sigma Aldrich	Lyophilized powder