Figure 6.

Loss of SirT3 in established human cancer cell lines increases tumor growth and is dependent on ROS. Tumor volume (a) and tumor mass (b) 24 days after subcutaneous injection of HCT116 scramble control and SirT3 knockdown cells in Nu/Nu mice with or without 40 m NAC supplementation in the drinking water. (c, d) Pictures of tumors from a and b after mice were euthanized. (e) Quantitative PCR for VEGF-A and SirT3 on RNA isolated from the tumors. Error bars are s.e.m. and * indicates a P value <0.05 with two-tailed Student's t-test.