Table 2.

Inhibitors of Human GAPDHS Identified from a Pilot Screen of the Prestwick Set

No.	Compound IDa	Name	Structure	% Inhibition in HTS	IC50 (µM)b	Hill Slope Valuec
1	PWK-433663	Propidium iodide		69.2	28	1.01
2	PWK-433331	Menadione		37.9	40% inhibition at 80 µM	1.10

^aPrestwick chemical identifier number.

^bFor IC₅₀ determinations, serial dilutions of compounds were tested starting at a high concentration of 80 µM. If 50% inhibition was not achieved at top dose, value is shown as % inhibition at 80 µM.

^cIC₅₀ and Hill slope values determined by XLfit software (ID Business Solutions).