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. Author manuscript; available in PMC: 2011 Jul 13.
Published in final edited form as: Nat Biotechnol. 2010 Feb 14;28(3):249–255. doi: 10.1038/nbt.1606

Figure 2. A nutrient sensitized screen to discover agents that shift energy metabolism.

Figure 2

(a) Schematic of the drug screen. MCH58 cells grown in 96 well plates in glucose or galactose containing media are exposed to a chemical library of 3695 compounds for 72 hours. The logarithm of the normalized cell number in glucose versus galactose serves as a summary statistic (Sglu/gal) for each compound.

(b) Results from a nutrient sensitized screen. Sglu/gal is plotted for top and bottom 250 compounds. Known oxidative phosphorylation (OXPHOS) inhibitors are highlighted in red and anti-neoplastic drugs are highlighted in blue.

(c) Secondary assays to evaluate compounds with modest yet positive Sglu/gal scores. The OCR/ECAR ratio of selected compounds is plotted against the compounds’ corresponding Sglu/gal score from panel (b). OCR and ECAR measurements were made on MCH58 cells grown in glucose and are normalized to cell viability. Compounds indicated by red symbols exhibited a statistically significant decrease in the OCR/ECAR ratio based on at least three independent replicates (P<0.05; two-sided t-test).