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. Author manuscript; available in PMC: 2011 Jul 13.
Published in final edited form as: Nat Biotechnol. 2010 Feb 14;28(3):249–255. doi: 10.1038/nbt.1606

Figure 5. Meclizine is cardioprotective in cellular and ex vivo models of cardiac ischemia.

Figure 5

(a) Protocol for the simulated ischemia-reperfusion (SIR) model.

(b) Viability of adult rat cardiomyocytes subjected to SIR, in the presence of indicated concentrations of meclizine (Mec), Atropine (Atrop), Pheniramine (Phenir), Pyrilamine (Pyril) and Scopolamine (Scopo).

(c) Respiration of cardiomyocytes following exposure to indicated concentrations of meclizine.

(d-e) Langendorff perfused rat hearts were subjected to 25 minutes of global ischemia followed by 2 hours of reperfusion. Meclizine treatment comprised infusion of 1μM (Mec) from a port above the aortic cannula for 20 minutes, followed by a 1 minute washout prior to ischemia. (d) Rate pressure product (heart rate × left ventricular developed pressure) is expressed as a % of the initial value throughout the ischemia-reperfusion (IR) protocol. (e) Following IR, hearts were stained with 2,3,5-Triphenyltetrazolium chloride (TTC) and infarct size was quantified. All data are means ± SEM from 4-6 individual experiments. (*P<0.05, ANOVA in panel d, Student’s t-test in panel e).