Skip to main content

View full-text article in PMC

. 2011 May 27;133(28):10892–10900. doi: 10.1021/ja202788q

Table 2. Apparent Kinetic Constants for Different Glycosynthases on α-Xyloglucosyl Fluoride Substrates.

	XXXGαF			XLLGαF
enzyme	K_M(app) (mM)	k_cat(app) (min^–1)	k_cat/K_M (mM^–1 min^–1)	K_M(app) (mM)	k_cat(app)(min^–1)	k_cat/K_M (mM^–1 min^–1)
HiCel7B E197S^a	8.2 ± 0.8	120 ± 10	15
PttXET16-34 E85A^b	1.6 ± 0.4	1.1 ± 0.1	0.66	3.8 ± 0.2	1.4 ± 0.04	0.38
TmNXG1 E94A^a	1.2 ± 0.2	2.6 ± 0.1	2.2	1.4 ± 0.2	8.2 ± 0.8	1.4
PpXG5 E323G	9.5 ± 2.7	73 ± 14	7.7	0.8 ± 0.09	28.9 ± 1.2	37

^a

Data from ref (24).

^b

Data from ref (25).