Figure 8.

CDDO and PGA₁ inhibit IL-1β-induced NF-κB transcriptional activity in human lung fibroblasts. Primary human lung fibroblasts were transfected with a luciferase reporter, then pretreated with 20 μM rosiglitazone (Rosi), 1 μM CDDO, 5 μM 15d-PGJ₂ (PGJ₂), 5 μM CAY10410 (CAY), or 15 μM PGA₁ and cotreated with of IL-1β for 24 hours as described. NF-κB-dependent luciferase activity was measured in lysates as described, normalized to protein concentration, and expressed as relative light units (RLU). NF-κB activation is significantly reduced in PPARγ ligand-treated fibroblasts compared to IL-1β alone (*P < .01). Results are mean ± standard deviation for quadruplicate wells and are representative of 2 independent experiments that yielded similar results.