Table 1.
Primer sequences
| Gene | Accession no. | Fragment | Forward primer | Reverse primer |
|---|---|---|---|---|
| QPCRs | ||||
| Slc4a4 | NM_018760 | 222–325 | TCCGACAAATCTGATGTGGA | TCCTCTCCCAAGATGAATCG |
| Slc4a8 | NM_021530 | 763–744 | CTGGACATGCGTGCAAGTAG | CTTTAACCCTCACGCTGTCG |
| Slc9a1 | NM_016981 | 1189–1170 | ATCCTTGTCTTCGGGGAGTC | TGCCCACAGAGTCATAGCTG |
| ActB | NM_007393 | 858–911 | GCTCTTTTCCAGCCTTCCTT | AGTTTCATGGATGCCACAGG |
| Multiplex PCRs | ||||
| Slc4a4 | NM_018760 | 76–697 | ATGTGTGTGATGAAGAAGAAGTAGAAG | GACCGAAGGTTGGATTTCTTG |
| ActB | NM_007393 | 305–877 | TGTTACCAACTGGGACGACA | AAGGAAGGCTGGAAAAGAGC |
| Nested PCRs | ||||
| Slc4a4 | NM_018760 | 359–577 | GGAACTCGATGAGCTTCTGG | ACCAGCTGTGGGAGAGAAGA |
| ActB | NM_007393 | 470–696 | AGCCATGTACGTAGCCATCC | TCTCAGCTGTGGTGGTGAAG |