Table 4.

Frequently Used Method for Identifying Lipid Raft Redox Signaling Platforms

Morphological observation of LR redox signaling platforms

Fluorescence confocal microscopy

Fluorescent resonance energy transfer

Flow cytometry or fluorescence activated cell sorter

Total internal reflection fluorescence microscopy

Fluorescence correlation and cross-correlation spectroscopy

Atomic force microscopy

Scanning ion conductance microscopy

Stimulated emission depletion

Biochemical characterization of LR redox signaling platforms

Floatation of detergent resistant membranes

Immunoblot analysis

Immunoprecipitation

Reactive oxygen species measurements

Electron spin resonance

Lucigenin-enhanced chemiluminescence

Dihydroethidium fluorescent spectrometric assay

Fluorescent dye intracellular trapping detection

HPLC analysis

Fluorometric or colorimetric assay of H2O2