Ca2+ transients recorded from 7+40- to 7+90-day dsRed+ ventricular hESC-CMs. (A) Representative tracings of Ca2+ transients. (B–E) Comparison of the basal [Ca2+]i (B), amplitude (C), maximum upstroke velocity (Vmax-upstroke, D), and maximum decay velocity (Vmax-decay, E) of Ca2+ transients in 40-day (n = 9) and 90-day (n = 10) dsRed+ ventricular hESC-CMs. *P < 0.05, **P < 0.01.