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Developmental changes of NCX current (INCX) in dsRed+ ventricular hESC-CMs. (A) The transcriptional expression of Na+/Ca2+ exchanger (NCX) 1, NCX2, and NCX3 in hESCs, hESC-ventricular (V) CMs, fetal VCMs (FV), and adult VCMs (AV). (B) Representative tracings of Ca2+ transients in 7+40- and 7+90-day dsRed+ cells before and after Ni+ treatment. (C) Protocol and typical tracings of INCX, and (D) I/V curve of INCX in 7+40- and 7+90-day dsRed+ cells. *P < 0.05, **P < 0.01.
