FIG. 2.

Expression of neural markers at Stg IV of human-induced pluripotent stem (hiPS) cells. (A) Neural progenitor markers Nestin (red) and Sox2 (green) were detected in many Stg IV hiPS cells, including cells inside rosettes. (B) Dopamine neurons in the midbrain (mDA) lineage markers Aldh1a1 and Lmx1a were expressed in Stg IV hiPS cells. Notice that there were 2 types of Aldh1a1 expressing cells: one was with long processes and inside rosettes or aggregates (arrowhead), the other was flat and outside aggregates (arrow). (C) Similar to human embryonic stem (hES) cells, mDA-specified hiPS cells labeled by Lmx1a also expressed TrkB on their surface. Dapi was used to counterstain all the nuclei in the field (A–C). Scale bar = 50 μm for A–C. (D–F) Higher magnification confocal images of A–C, respectively.