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Detection of an immune response to HSV-Tk in patient UPN 919. After coculture with SFCMM-3-transduced donor cells (straight line) or allogeneic cells (dashed line), patient and donor PBMCs were tested against the same stimulators and untransduced donor cells in a standard 51Cr release assay. Although patient effectors showed a weaker response to allogeneic targets as compared with donor effectors, only patient T cells showed a specific lytic response to transduced lymphocytes.
