hESC proliferation and OCT4 expression for cells grown in SDEC-, MEF-, WI38-, and DET-conditioned medium on Matrigel. (A) Population doubling (mean ± SE, N = 3) of hESC over 3 weeks. (B) Percentage of OCT4-positive cells (mean ± SE, N = 3) after 3 weeks. (C) Immunofluorescent staining of hESC with OCT4 after 3 weeks. Nuclei stained with DAPI. Scale bar = 50 μm (magnification × 400). Significance: *P < 0.05 and ***P < 0.001. DAPI, 4′,6-diamidino-2-phenylindole; DET, Detroit 551; hESC, human embryonic stem cells; MEF, mouse embryonic fibroblast; SE, standard error.