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. 2010 Apr 1;19(12):1923–1935. doi: 10.1089/scd.2009.0326

FIG. 4.

FIG. 4.

Expression of sFRP-1 and PTGS2 and prostaglandins by DET, WI38, and SDEC cell types. Quantitative RT-PCR was done to determine the fold difference in mRNA expression level (mean ± SE, N = 3) for (A) sFRP-1 and (B) PTGS2. mRNA levels were normalized using PPIA mRNA (housekeeping gene). Semiquantitative western blots were done on (C) intercellular PTGS2 (using β-actin to control for protein loading) and (D) sFRP-1 recovered from CM (180 mL from SDEC and DET, 150 mL from WI38). sFRP-1 recovered from MEF CM (180 mL) was also included in this western blot. The indicated amounts of recombinant human sFRP-1 were included for comparison. (E) Levels (mean ± SE, N = 3, ng/mL) of 6-k-PGF1α (black) and PGE2 (white) in CM from the 3 human cell types, MEF CM, and unconditioned human embryonic stem cells medium (BASE). Significance: **P < 0.01 and ***P < 0.001. CM, conditioned medium; DET, Detroit 551; MEF, mouse embryonic fibroblast; PGE2, prostaglandin E2, PTGS2, prostaglandin-endoperoxide synthase 2; RT-PCR, reverse transcription polymerase chain reaction; sFRP-1, secreted frizzled-related protein; 6-k-PGF1α, 6-keto-prostaglandin F1α; SE, standard error.