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Cell proliferation assay of H9 human embryonic stem cells grown on COL or MAT matrices in SDEC-conditioned medium supplemented with various concentrations of sFRP-1. Proliferation of control (no added sFRP-1) was set to 100% and the percent of control proliferation (mean ± standard error, N = 4) for each sFRP-1 concentration is reported. Significance: *P < 0.05, **P < 0.01, and ***P < 0.001. MAT, Matrigel; sFRP-1, secreted frizzled-related protein.
